Introduction. Nepeta ispahanica Boiss. (Lamiaceae), is an endemic herbaceous plant, growing wild in Iran. Some of Nepeta species are used as medicinal plants in Iranian folk medicine. Because of biological activity of essential oils and in the course of studying on Nepta species, the essential oil of N. ispahanica is analyzed.Methods. Aerial parts of the wild plants of N. ispahanica were collected from Kolah-Ghazi in the south of Isfahan. The essential oil was obtained by hydrodistillation. The oil was analyzed by GC/MS method. Results. The aerial parts of N. ispahanica contained 0.4% (v/w) pale yellow essential oil. The major components were: 1,8-cineole (45.8%), ß-pinene (8.9%), 4aα,7α,7aα - nepetalactone (6.2%), α-terpineol (4.3%) and trans-ß-ocimene (3.3%).